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Botany Butterfly Farming Gardening Horticulture Monarch (Danaus plexippus) Queen (Danaus gilippus)

DIY Guide: How to Test Milkweed Plants for O.E. Using a 40X Student-Grade Microscope

Ophryocystis elektroscirrha (O.E.) is a protozoan parasite that can infect Monarch and Queen butterflies. The parasite’s spores often reside on milkweed leaves, the primary food source for Monarch larvae.

View of Monarch Butterfly scales, which are the larger, apple seed or feather shaped objects, heavily infected with O.E., the spores of which are the smaller, dust-like particles. The inset photo is 100X magnification of the football-shaped O.E. spores.

In this guide, we’ll walk you through the process of testing milkweed leaves for the presence of O.E. spores using just a 40X student-grade microscope.

100X magnification of scales and spores.

Materials Needed

  • 40X Student-Grade Microscope
  • Milkweed leaves
  • Transparent tape (Scotch tape or similar)
  • Glass microscope slides
  • Tweezers
  • Latex or nitrile gloves
  • Notebook and pen for recording observations

Safety Precautions

  • Always handle the microscope carefully.
  • Wear gloves to avoid contamination.
  • Dispose of used materials properly.

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