Step 5: Examine Under the Microscope

1. Place the slide under the microscope.
2. Adjust the microscope to the 40X magnification setting. 100X is better, if available.
3. Focus the microscope until the leaf material becomes clear.
4. Scan the slide slowly, looking for O.E. spores, which will appear as small, oval, and darkly colored objects, much like American footballs.

Step 6: Record Observations

1. Keep a notebook handy to jot down what you see.
2. If possible, capture images through the microscope for documentation.
3. Note the density and distribution of the spores, if present.

Step 7: Dispose of the Sample

1. Once done, carefully remove the slide from the microscope stage.
2. Dispose of the tape and slide in a proper waste container.

Step 8: Clean Up

1. Turn off the microscope and cover it.
2. Dispose of your gloves.
3. Clean your workspace.

Conclusion

Testing for O.E. spores on milkweed leaves is crucial for understanding the health of your local Monarch and Queen butterfly populations. Although a 40X student-grade microscope may not offer the highest resolution, it is sufficient for a basic O.E. test. This DIY guide makes the process simple enough for anyone to carry out, contributing to citizen science and the conservation of these beautiful creatures… one day at a time!

Milkweed Seeds

Seeds for growing plants in the Milkweed genus (Asclepias); required for all Milkweed butterflies, like the Monarch and Queen.

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